Part:BBa_K2800027

Ptet promoter

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Biology:

    This part is inducible promoter Ptet which is induced by tetracycline. This is the basic principle of Tet induced expression system, it is to regulate the conformation of proteins by inducer such as Tet, thus controlling the expression of target proteins. When there is no tetracycline, TetR will bind to TetO to block downstream expression of resistant genes. When tetracycline is added, it will bind to TetR and change its conformation, then TetR is separated from TetO, thereby relieving the inhibition of resistance genes.

Fig.1 The principle of Tet and TetR/TetO system.

Characterize: HUBU-WUHAN 2019

Tetracycline is not only an inducer of inducible promoter Ptet , but also a spectral antibiotic. Tetracycline antibiotics are protein synthesis inhibitors. They inhibit the initiation of translation in variety of ways by binding to the 30S ribosomal subunit, which is made up of 16S rRNA and 21 proteins. They inhibit the binding of aminoacyl-tRNA to the mRNA translation complex.

Fig.2 The mechanism of Tc.

Fig.3 .The effects of different concentrations of tetracycline on the growth of Zymomonas mobilis.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]